Immunohistochemical Analysis of Targeted Nanoparticles in Brain Tissue After Traumatic Grain Injury

Abstract

This project primarily focused on the analysis of immunohistochemical mice brain samples post-traumatic brain injury (TBI) to assess the targeting and retention of nanoparticles (NPs) conjugated with targeting and control peptides. The tissue sections analyzed were stained for cell nuclei (DAPI), blood vessels (CD31), neurons (Neun), microglia (Iba1), and astrocytes (GFAP) are used in addition to targeted fluorescent NPs. The HALO imaging software was utilized to obtain the area percentage of each marker in each of the following brain structures; cerebral cortex, thalamus, hippocampus, and corpus callosum of the ipsi- and contralateral hemispheres to the injury site.

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