Effects of Physiological Glucose and Oxygen Conditions on Human Neural Organoid Development

Abstract

Standard culture conditions use non-physiological glucose (20-25mM) and oxygen (20%) levels that may alter neural development. This project investigated how aligning culture conditions with physiological levels (5mM glucose, 8% oxygen) affects neural organoid development. We found that physiological conditions promote human specific features of cortical development, including outer radial glia expansion and upper-layer neuron production, while constraining early deep layer neurogenesis.

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